Infanta Isabella of Castile, Duchess of York (c. 1355 – 23 December 1392) was a daughter of King Peter of Castile and María de Padilla. She was a younger sister of Constance, Duchess of Lancaster.

In 1372, sometime between the 1 March and 30 April, Isabella married Edmund of Langley, 1st Duke of York, fourth son of Edward III of England and Philippa of Hainault at Wallingford. As a result of her marriage, she became the first of a total of eleven women who became (as a courtesy by marriage to their husbands) Duchess of York. They had three children:

 Edward of Norwich, 2nd Duke of York (1373 – 25 October 1415).
 Constance of York (1374 – 29 November 1416). Married Thomas le Despenser and was mother of Isabel le Despenser, Countess of Worcester and Warwick.
 Richard of Conisburgh, 3rd Earl of Cambridge (1375 – 5 August 1415).

She was named a Lady Companion of the Order of the Garter in 1378. Isabella died 23 December 1392 and on 14 January 1393 was buried in Kings Langley Manor House in Hertfordshire, England.
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