Maria Leopoldine (6 April 1632, Innsbruck – 7 August 1649) was Holy Roman Empress as the spouse of Ferdinand III, Holy Roman Emperor.
She was a daughter of Leopold V, Archduke of Austria and Claudia de' Medici and empress consort of Ferdinand III, Holy Roman Emperor. On 2 July 1648 in Linz, she married Ferdinand. She bore him one child, Karl Josef of Austria (7 August 1649), who died in his mid-teens. Maria Leopoldine died in childbirth 7 August 1649, and is buried in tomb 21 in the Imperial Crypt in Vienna.